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The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion. JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype. The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. [PubMed: 9753063]
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C
Subculturing:
Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10(4) to 4 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 2.5 X 10(6) cells/ml.
92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365 16172573: Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697 16172575: Jeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063
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