久久频道毛片免费不卡片,四虎精品影视,欧美日韩中文制服有码,99福利网,中文字幕第七页,在线国产69自拍视频,亚洲欧美在线播放,亚洲一区欧美二区 

    
        
        
        
            
        
                
        
                    
                
        
                
                
        
                    
        
                        
        
                            
        
                                
        
                                    
                                
        
                                
        
                                    
                                    
        
                                        
        
                                            
        
                                                
        
        
        
        
        
            
        
                
        
                    
                    
                    
                    
                    
                
        
                
        
                
        
                    
        
                        
        
                            產品詳情
                        
        
                        
        
                            
        
                                
                                
                                
                                
                            
        
                            
        
    
        
    
        
        
        
    
        
            
        

                
        
                    
                    
          
                          
        • 產品名稱：Cos-7 非洲綠猴sv40轉化的腎細胞
          • 
                          
        • 產品型號：CRL-1651
          • 
                          
        • 產品廠商：美國標準生物品收藏中心（ATCC）
          • 
                          
        • 產品價格：0
          • 
                          
        • 折扣價格：0
          • 
                          
        • 產品文檔：
                          
          • 




                    
        
                    
          
                           
        • 
                             
          
                               
                             
          
                         
          • 
                    
        
                     
        
                    你添加了1件商品 查看購物車
                    
        

                
        
            
        
    
        

    
        
        
        
            簡單介紹：
        
        
        
        
            CRL-1651 Cos-7 非洲綠猴sv40轉化的腎細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件！
        
        
    
        

    
        
        
        
            詳情介紹：
        
        
        
        
            CRL-1651 Cos-7 非洲綠猴sv40轉化的腎細胞
        

        
	
		
        
			
				ATCC® Number: 
			
			
				CRL-1651?    
			
			
				Price: 
			
			
				$269.00
			
		
        
		
        
			
				
        
					
						
        
							
								Designations: 
							
							
								COS-7
							
						
        
						
        
							
								Depositors: 
							
							
								 Y Gluzman 
							
						
        
						
        
							
								Biosafety Level:
							
							
								2 [Cells Contain SV-40 viral DNA sequences ]
							
						
        
						
        
							
								Shipped: 
							
							
								frozen 
							
						
        
						
        
							
								Medium & Serum: 
							
							
								See Propagation
							
						
        
						
        
							
								Growth Properties:
							
							
								adherent
							
						
        
						
        
							
								Organism:
							
							
								Cercopithecus aethiops
							
						
        
						
        
							
								Morphology:
							
							
								fibroblast
        
CRL-1651 Cos-7 非洲綠猴sv40轉化的腎細胞
        
        
							        		
						
        
						
        
							
								Source:
							
							
								Organ: kidney 
        
Cell Type: SV40 transformed        
							        		
						
        
						
        
							
								Cellular Products:
							
							
								T antigen
							
						
        
						
        
							
								Permits/Forms:
							
							
								In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
							
						
        
						
        
							
								 
							
						
        
						
        
							
								Applications:
							
							
								transfection host (Nucleofection technology from Lonza
        
Roche FuGENE® Transfection Reagents)        
							        		
						
        
						
        
							
								Virus Susceptibility:
							
							
								SV40 (lytic growth); SV40 tsA209 at 40C; SV40 mutants with deletions in the early region
							
						
        
						
        
							
								Comments:
							
							
								This is an African green monkey kidney fibroblast-like cell line suitable for transfection by vectors requiring expression of SV40 T antigen. This line contains T antigen, retains complete permissiveness for lytic growth of SV40, supports the replication of ts A209 virus at 40C, and supports the replication of pure populations of SV40 mutants with deletions in the early region.The line was derived from the CV-1 cell line (ATCC ® CCL-70?) by transformation with an origin defective mutant of SV40 which codes for wild type T antigen.
							
						
        
						
        
							
								Propagation: 
							
							
								ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
        
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
        
Temperature: 37.0°C        
							        		
						
        
						
        
							
								Subculturing: 
							
							
								Protocol: 
								
          
									
        1. 
										Remove and discard culture medium.
									
          2. 
									
        2. 
										Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
									
          3. 
									
        3. 
										Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
          
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
									
          4. 
									
        4. 
										Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
									
          5. 
									
        5. 
										Add appropriate aliquots of the cell suspension to new culture vessels.
									
          6. 
									
        6. 
										Incubate cultures at 37°C.
									
          7. 
								
        
        
							        		
						
        
					        
				
        
			        		
		
        
	        

        
        
        
    
        

        
 
    
        

        

                            
        
    
        
    
        
        
        

  
        


          
        
              姓名：
              
        
                  
              
        
          
        
          
        
              電話：
              
        
                  
              
        
          
        
          
        
                  Email：
                  
        
                      
                  
        
              
        
          
        
              您的需求：
              
        
                  
              
        
          
        
          
        
               
              
        
                  
                  
              
        
          
        

  
        
  

        

    
        

        

                        
        
                    
        
                
        
            
        
        
        
    
        
                                            
        
                                        
        
                                    
        
                                
        
                            
        
                        
        
                    
        
                
        
                
            
        
		 		
        滬公網安備 31010902002429號
        
		 	
        
		 
        
        
        
    
        
 


主站蜘蛛池模板：
国产激情一区二区三区在线蜜臀|
国产人成在线免费视频|
静宁县|
日韩秘 无码一区二区三区|
新泰市|
91人妻无码成人精品一区91|
日韩精品一区二区三区蜜臀|
亚洲国产精品无码java|
婷婷综合久久中文字幕|
国产av一区二区三区东北熟女|
一区二区特别黄色大片|
日本欧美v大码在线|
国产精品美女久久久久av爽李琼|
欧美亚洲第一页|
男女性搞视频网站免费|
精品中文字幕手机在线|
精品国产一区二区三区香|
日韩欧美在线观看一区二区视频
|
日本a在线天堂|
亚洲一区二区观看网站|
免费吃奶摸下激烈视频青青网|
免费国人成人自拍视频|
亚洲中文字幕在线精品一区|
熟女一区二区三区视频|
亚洲AV专区一专区二专区三|
超碰日韩AV在线|
国产高潮自拍视频在线观看
|
亚洲AV无码成人精品区日韩密殿|
山东|
久久人妻av无码中文专区|
中文字幕无字幕加勒比|
久久久人人人婷婷色东京热|
日韩人妻无码精品二专区|
蜜桃视频在线网站免费|
欧乱色国产精品兔费视频|
亚洲精品第1页|
亚洲av五月天天堂网|
久久精品成人免费观看97|
亚洲不卡av一区二区三区|
国产色无码专区在线观看|
婷婷激情六月|