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        TOV-21G 人卵巢癌細(xì)胞

        日期:2025-12-23 15:19
        瀏覽次數(shù):1868
        摘要: Designations: TOV-21G Depositors: University of Montreal Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Homo sapiens deposited as human Morphology:
        Designations: TOV-21G
        Depositors:  University of Montreal
        Biosafety Level: 1
        Shipped: frozen
        Medium & Serum: See Propagation
        Growth Properties: adherent
        Organism: Homo sapiens deposited as human
        Morphology: epithelial

        Source: Organ: ovary 
        Tumor Stage: grade 3, stage III 
        Disease: primary malignant adenocarcinoma; clear cell carcinoma
        Cellular Products: keratin
        Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
        Applications: Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24.
        Tumorigenic: Yes
        Oncogene: p53 + (wild type)
        DNA Profile (STR): Amelogenin: X 
        CSF1PO: 13,15 
        D13S317: 11,12 
        D16S539: 10,12 
        D5S818: 12,13 
        D7S820: 12 
        THO1: 7,9.3 
        TPOX: 8,11 
        vWA: 17
        Cytogenetic Analysis: 47, XX, +10 [49408 ]
        Age: 62 years
        Gender: female
        Comments: This cell line was initiated in October of 1991 from a patient of French-Canadian descent with no family history of ovarian cancer. 
        Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24.
        Propagation: ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:
      1. fetal bovine serum to a final concentration of 15%

      2. Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
        Temperature: 37.0°C
        Subculturing: Protocol:
        1. Remove and discard culture medium.
        2. Briefly rinse the cell layer with 0.05% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
        3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
          Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
        4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
        5. Add appropriate aliquots of the cell suspension to new culture vessels.
        6. Incubate cultures at 37?C.

        Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended 
        Medium Renewal: Every 3 to 4 days
        Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
        Storage temperature: liquid nitrogen vapor phase
        Related Products: recommended serum:ATCC 30-2020
        References:

        42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998
        49408: Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

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        CTLA4 Ig-24 中國(guó)倉(cāng)鼠卵巢細(xì)胞 TOV-21G 人卵巢癌
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        CHO/dhFr- 倉(cāng)鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷 SK-OV-3 人卵巢癌細(xì)胞TOV-21G 人卵巢癌細(xì)胞TOV-21G 人卵巢癌細(xì)胞

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